QUANTIFICATION OF ENZYMES AND HISTOLOGY CHANGES BELONGINGS TO THE PINK BOLL WORM PECTINOPHORA GOSSYPIELLA (SAUNDERS) (LEPIDOPTERA: GELECHIIDAE) EXPOSED TO SOME INSECTICIDE TOXICITIES

Abstract

The direct effects of insecticide class toxicities on many numbers of insect body enzymes, is the strong asset to measure the biological effects. Then much quantity of P.gossypiella larvae collected from several fields were treated with sublethal concentration equal LC₅₀ values of some recommended insecticides two of organophosphates and pyrethroids in addition to orange oil were selected and evaluated about most enzymes specific detoxification mechanisms exist and its mid gut histological changes of effected bodies were investigated. Data of enzyme investigations shows that cypermethrin was recorded high value of AChE activity was 305 (ug AchBr/min/g.b.wt), followed by malathion was 268 then profenofos was 243 and lambdacyhalothrin was 217.3. Nevertheless, orange oil was recorded high value in GST activity monitoring followed by cypermethrin, malathion then profenophos. Moreover, orange oil recorded the least value of alkaline phosphatase, alpha, beta esterases activity and the total protein. The highest values were cypermethrin in alkaline phosphatase, profenofos in alpha and beta esterase and the total protein. The percentage of inhibition of Enzymes activity compared with the control were calculated. Histological observations in all slides were in larvae mid-gut were detected the differences between insecticide toxicities.